Routine sequencing

Full length amplicon sequencing - 15 EUR*

For amplicons starting from 100 bp

16S / ITS sequencing - from 35 to 135 EUR*

price depends on number of reads

Whole plasmid sequencing - 15 EUR*

For plasmids from 1,5 to 30 kb.

dsDNA bacteriophage genome sequencing - 150 EUR*

Phage Direct - 160 EUR*

100 €/sample for ≥10 phages

*prices do not include VAT

Small genome sequencing - 100 EUR*

For bacterial, fungal genomes up to 12 Mb

Additional bioinformatics analysis - 50 EUR/hour*

Upon request

Custom sequencing projects

Upon request

110 €/sample for ≥10 phages

Services

Whole plasmid sequencing

Full length amplicon sequencing

Long read whole plasmid sequencing with automatic assembly, annotations, variant calling.

Long read PCR amplicon sequencing with automatic assembly, annotations and variant calling.

Fast and highly accurate whole plasmid sequencing for plasmids from 1,5 kb to 30 kb;
No primers;
No prior information about the plasmid;
Ideal to resolve plasmid recombination and duplications.

Fast and highly accurate sequencing for all amplicons starting at
100 bp;
No primers;
No prior information about the amplicon;
Reads are not fragmented and fully capture the sequence resolving variants.

Sample requirements:

• 15 µl of 40 ng/ul purified plasmid DNA.

Sample requirements:

• 10 µl of 30-40 ng/ul purified PCR amplicon >5kb in length.

• 10 µl of 10-20 ng/ul purified PCR amplicon, >1kb <5kb in length.

• 10 µl of 5 ng/ul purified PCR amplicon, >200 bp <1kb in length.

• 10 µl of 1 ng/ul, for amplicons <200 bp.

We highly recommend using column purification method and eluting the plasmid with water or a buffer without EDTA.

We highly recommend using column purification method and eluting the plasmid with water or a buffer without EDTA.

Small genome sequencing

Bacteriophage genome sequencing

Long read sequencing of bacterial or fungal genome up to 12 Mb with an automatic assembly and annotation.

Fast and highly accurate genome sequencing;
Chromosomes and plasmids resolved;
Automatic genome annotation;

Sample requirements:

• 20 µl of 40 ng/ul high purity, high molecular weight genomic DNA.

We highly recommend using column purification method and eluting the plasmid with water or a buffer without EDTA.

Long read sequencing of dsDNA bacteriophage genome up to 250 Kb with automatic assembly and annotation.

Fast and highly accurate genome sequencing;
PCR based library preparation;
Automatic assembly and genome annotation;

Sample requirements:

• 10 µl of 10 ng/ul high purity, high molecular weight bacteriophage genomic DNA.

We highly recommend using column purification method and eluting the plasmid with water or a buffer without EDTA.

Custom sequencing projects

We offer other DNA and direct RNA sequencing solutions not limited by small standardized services.

The services include:

• Whole genome sequencing of large eukaryotic genomes.

• Transcriptomics: cDNA and direct RNA sequencing.

• Metagenomic DNA sequencing.

• DNA and RNA modification sequencing.

Please contact us at orders@seqvision.com with your specific needs.

Phage Direct sequencing

Oxford Nanopore based sequencing of DNA suspension with automatic assembly and annotation.

Up to 500 kb genome size;
Quickest phage sequencing to data;
DIRECT sequencing from phage suspension;
No DNA purification on your side;
Ideal for screening and isolating new phages;
Ideal for analysing phage mutants.

Sample requirements:

• 30-50 µl of high titer phage suspension (>109 pfu/ml)

Phage suspension should not contain any bacteria or cell debris.

16S sequencing

Long read sequencing of the full 16S gene region with automatic analysis.

No fragmentation;
PCR included;
Full report;
Sample purification option.

Sample requirements:

• 10 µl of 10 ng/ul high purity, DNA sample.

We recommend eluting in water or a buffer without EDTA.

ITS sequencing

Long read sequencing of the full ITS1-ITS4 region with automatic analysis.

No fragmentation;
PCR included;
Full report;
Sample purification option.

Sample requirements:

• 10 µl of 10 ng/ul high purity, DNA sample.

We recommend eluting in water or a buffer without EDTA.